The toxic effect of Vu-Defr, a defensin from Vigna unguiculata seeds, on Leishmania amazonensis is associated with reactive oxygen species production, mitochondrial dysfunction, and plasma membrane perturbation
Autor: | Edésio José Tenório de Melo, Laís Pessanha de Carvalho, André de Oliveira Carvalho, Géssika Silva Souza, Valdirene Moreira Gomes |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Immunology Antimicrobial peptides Applied Microbiology and Biotechnology Microbiology law.invention Defensins Vigna Cell membrane 03 medical and health sciences Anti-Infective Agents law Genetics medicine Animals Molecular Biology Defensin Leishmania Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species biology Plant Extracts Chemistry Cell Membrane General Medicine biology.organism_classification Recombinant Proteins In vitro 030104 developmental biology medicine.anatomical_structure Mechanism of action Seeds Recombinant DNA medicine.symptom Reactive Oxygen Species |
Zdroj: | Canadian Journal of Microbiology. 64:455-464 |
ISSN: | 1480-3275 0008-4166 |
DOI: | 10.1139/cjm-2018-0095 |
Popis: | Plant defensins are plant antimicrobial peptides that present diverse biological activities in vitro, including the elimination of Leishmania amazonensis. Plant defensins are considered promising candidates for the development of new drugs. This protozoan genus has great epidemiological importance and the mechanism behind the protozoan death by defensins is unknown, thus, we chose L. amazonensis for this study. The aim of the work was to analyze the possible toxic mechanisms of Vu-Defr against L. amazonensis. For analyses, the antimicrobial assay was repeated as previously described, and after 24 h, an aliquot of the culture was tested for viability, membrane perturbation, mitochondrial membrane potential, reactive oxygen species (ROS) and nitric oxide (NO) inductions. The results of these analyses indicated that after interaction with L. amazonensis, the Vu-Defr causes elimination of promastigotes from culture, membrane perturbation, mitochondrial membrane collapse, and ROS induction. Our analysis demonstrated that NO is not produced after Vu-Defr and L. amazonensis interaction. In conclusion, our work strives to help to fill the gap relating to effects caused by plant defensins on protozoan and thus better understand the mechanism of action of this peptide against L. amazonensis. |
Databáze: | OpenAIRE |
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