Protective Effects of 5-HT1A Receptor Inhibition and 5-HT2A Receptor Stimulation Against Streptozotocin-Induced Apoptosis in the Hippocampus
| Autor: | Alireza Komaki, Siamak Shahidi, Nasrin Hashemi-Firouzi, Simin Afshar, Sara Soleimani Asl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Agonist endocrine system diseases medicine.drug_class hippocampus Hippocampus Pharmacology 5-HT2A receptor Serotonergic streptozotocin 03 medical and health sciences 0302 clinical medicine medicine 5-HT1A receptor rat Receptor business.industry apoptosis nutritional and metabolic diseases General Medicine Receptor antagonist Streptozotocin 030104 developmental biology nervous system Apoptosis Original Article business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | The Malaysian Journal of Medical Sciences : MJMS |
| ISSN: | 2180-4303 1394-195X |
| Popis: | Introduction: Intracerebroventricular administration of streptozotocin (icv-STZ) induced apoptosis changes in neurons similar to Alzheimer's disease. The serotonergic system via its receptor involved in survival of neurons. The present study examined the ability of selective 5-HT1A receptor antagonist (NAD-299) and 5-HT2A receptor agonist (TCB-2) to attenuate the apoptosis caused by the icv-STZ in the rat. Methods: The icv-STZ (3 mg/kg, 10 μL, twice) induced neuronal loss in the hippocampus of adult male rats. Animals were divided into naive control, sham-operated, STZ+saline (1 μL, icv), STZ+NAD-299 (5 μg/μL, icv), STZ+TCB-2 (5 μg/μL, icv), and STZ+NAD-299+TCB-2 (5 μg/μL of any agent, icv) groups. Following the 35 days’ treatment period, neuronal apoptosis was detected using the Tunnel. Cells with morphological features of apoptotic cell were contended by microscopy. Results: TCB-2 and NAD-299 administration decreased number of apoptotic neurons in the treatment group compared with the STZ group. Combined treatment of STZ rat with NAD+TCB more decreased number of apoptotic cells in compare to TCB-2 or NAD-299 treated STZ groups. Conclusion: Treatment with 5-HT1A receptor antagonist or 5-HT2A receptor agonist diminished apoptosis. The beneficial effect of 5HT1A receptor inhibition was potentiated with activation of 5-HT2A receptor in prevention of apoptosis in hippocampus. © Penerbit Universiti Sains Malaysia. |
| Databáze: | OpenAIRE |
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