Anti-proliferative and immunomodulatory activities of fractions from methanol root extract of Abrus precatorius L
| Autor: | Almas Jabeen, Funmilayo D. Onajobi, Emeka Emmanuel Okoro, Sidrah Shams, Omolaja Osoniyi, M. I. Choudhary |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
DPPH
medicine.medical_treatment Cytotoxicity Ethyl acetate lcsh:Medicine lcsh:RX1-681 Pharmacology HeLa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer lcsh:Homeopathy Abrus precatorius medicine 030304 developmental biology General Environmental Science 0303 health sciences Tumor necrosis factor oxidative burst biology lcsh:R biology.organism_classification Respiratory burst Cytokine chemistry 030220 oncology & carcinogenesis Cancer cell Cervical cancer General Earth and Planetary Sciences Cytokines |
| Zdroj: | Clinical Phytoscience, Vol 5, Iss 1, Pp 1-9 (2019) BASE-Bielefeld Academic Search Engine |
| ISSN: | 2199-1197 |
| Popis: | Background Abrus precatorius possesses various therapeutic properties including anticancer potentials. This study evaluated the anti-proliferative activities of fractions of methanol root extract of A. precatorius on breast and cervical cancer cells and their immunomodulatory effect. Phytochemical screening was done by FTIR and GCMS. In vitro anti-proliferative effect was evaluated on human breast cancer (AU565) and cervical cancer (HeLa) cells and on murine fibroblast (NIH 3 T3) cells. Antioxidant activity was performed via DPPH radical scavenging assay. The immunomodulatory potential of fractions was evaluated by inhibition of phagocytes oxidative burst (ROS), Nitric oxide (NO) and proinflammatory cytokine TNF-α. Results A. precatorius fractions showed different chemical groups and were somewhat selective in antiproliferative activity against studied cancer cells. Ethyl acetate fraction showed the most significant antiproliferative activity with IC50 values of 18.10 μg/mL and 11.89 μg/mL against AU565 and HeLa cells respectively. Hexane fraction significantly (p 50 18.24 ± 0.16 μg/mL), whereas aqueous fraction showed mild inhibition (IC50 46.46 ± 0.14 μg/mL) on AU565 cell proliferation. All fractions showed no cytotoxicity against NIH-3 T3 murine fibroblast normal cells. All fractions showed potent and significant (p Conclusions The antiproliferative activities and suppressive effect on the phagocytic oxidative burst, NO and proinflammatory cytokine might be due to the synergistic actions of bioactive compounds especially flavonoids present in the assayed fractions and therefore, suggest chemotherapeutic use of A. precatorius in cancer treatment. |
| Databáze: | OpenAIRE |
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