Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells
| Autor: | Kun Qian, Zhongwei Du, Xiaofen Zhong, Frank Fan, Hong Chen, Su-Chun Zhang, Cindy Tzu-Ling Huang, Huisheng Liu, Jianfeng Lu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pluripotent Stem Cells
Cell type Cell Culture Techniques Neuromuscular Junction General Physics and Astronomy Biology General Biochemistry Genetics and Molecular Biology Article Cell therapy 03 medical and health sciences 0302 clinical medicine Neural Stem Cells medicine Humans Progenitor cell Induced pluripotent stem cell 030304 developmental biology Progenitor Motor Neurons 0303 health sciences Multidisciplinary General Chemistry Motor neuron Embryonic stem cell 3. Good health Cell biology medicine.anatomical_structure Cell culture Immunology 030217 neurology & neurosurgery |
| Zdroj: | Nature communications |
| ISSN: | 2041-1723 |
| Popis: | SUMMARY Human pluripotent stem cells (hPSCs) have opened new opportunities for understanding human development, modeling disease processes and developing new therapeutics. However, these applications are hindered by low-efficiency and heterogeneity of target cell types differentiated from hPSCs, such as motor neurons (MNs), as well as our inability to maintain the potency of lineage committed progenitors. Here, by using a combination of small molecules that regulate multiple signaling pathways, we develop a method to guide human embryonic stem cells to a near-pure population (>95%) of motor neuron progenitors (MNPs) in 12 days, and an enriched population (>90%) of functionally mature MNs in an additional 16 days. More importantly, the MNPs can be expanded for at least 5 passages so that a single MNP can be amplified to 1×104. This method is reproducible in human induced pluripotent stem cells and is applied to model MNdegenerative diseases and in proof-of-principle drug screening assays. |
| Databáze: | OpenAIRE |
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