AFM Imaging of RGD Presenting Synthetic Extracellular Matrix Using Gold Nanoparticles
| Autor: | Susan X. Hsiong, Peter H. Cooke, Maria Ericsson, David J. Mooney, Hyunjoon Kong, Marshall L. Fishman |
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| Rok vydání: | 2008 |
| Předmět: |
Polymers and Plastics
Alginates Biotin Metal Nanoparticles Nanoparticle Biocompatible Materials Bioengineering Nanotechnology macromolecular substances Conjugated system Microscopy Atomic Force Biomaterials Microscopy Electron Transmission Tissue engineering Materials Chemistry Tissue Scaffolds Chemistry technology industry and agriculture Biomaterial Hydrogels Adhesion Extracellular Matrix Colloidal gold Biotinylation Self-healing hydrogels Microscopy Electron Scanning Gold Streptavidin Oligopeptides Biotechnology |
| Zdroj: | Macromolecular Bioscience. 8:469-477 |
| ISSN: | 1616-5195 1616-5187 |
| Popis: | Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies. |
| Databáze: | OpenAIRE |
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