Polymorphic mononuclear neutrophils CD64 index for diagnosis of sepsis in postoperative surgical patients and critically ill patients
| Autor: | Pamela M.J. McLaughlin, Bert N. Nienhuis, Jan W. Smit, Bert Loef, Jeroen H. Gerrits |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Neutrophils Critical Illness Clinical Biochemistry Blood Sedimentation Severity of Illness Index Gastroenterology law.invention Sepsis Leukocyte Count law Internal medicine Intensive care Leukocytes medicine Humans Eosinopenia Postoperative Period Aged Aged 80 and over CD64 Bacteria medicine.diagnostic_test business.industry Receptors IgG Biochemistry (medical) General Medicine Middle Aged medicine.disease Intensive care unit Systemic Inflammatory Response Syndrome Systemic inflammatory response syndrome Intensive Care Units C-Reactive Protein ROC Curve Erythrocyte sedimentation rate Immunology Absolute neutrophil count Female business Granulocytes |
| Zdroj: | Clinical Chemistry and Laboratory Medicine (CCLM). 51:897-905 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm-2012-0279 |
| Popis: | Background: Surface neutrophil CD64 expression is upregulated in patients with bacterial infection. As it was suggested that the CD64 index could be used to detect sepsis in hospitalized patients, we questioned whether the CD64 index could discriminate between septic patients and postoperative surgical patients, defined as systemic inflammatory response syndrome (SIRS), both admitted at the intensive care unit (ICU). Furthermore, we wondered whether the CD64 index was an improved diagnostic compared to standard assays used at the laboratory. For this, outclinic (OC) patients were included as controls. Methods: The Leuko64™ assay was used to determine the CD64 index in residual EDTA blood samples from selected septic patients (n=25), SIRS patients (n=19), and OC patients (n=24). Additionally, WBC count, neutrophilic and eosinophilic granulocyte count, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were measured simultaneously. Results: The CD64 index was higher in septic patients compared to both the SIRS and OC group (p Conclusions: A high CD64 index was found in septic intensive care patients, while a low CD64 index was observed in OC and SIRS patients, demonstrating that the CD64 index can be used for routine diagnostics in the ICU setting. |
| Databáze: | OpenAIRE |
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