Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells: improving bioanalysis using electrochemistry
| Autor: | Claudio Parolo, Arben Merkoçi, Carme Nogués, Helena Montón, Antonio Aranda-Ramos |
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| Přispěvatelé: | Generalitat de Catalunya, European Commission, Ministerio de Ciencia e Innovación (España) |
| Rok vydání: | 2015 |
| Předmět: |
Bioanalysis
Novel techniques Surface Properties Confocal Drug development Apoptosis Phosphatidylserines Flow cytometry Cell Line chemistry.chemical_compound Annexin Microscopy Quantum Dots medicine Apoptotic cells Humans Confocal laser scanning microscopy General Materials Science Dimethyl Sulfoxide Annexin A5 Phosphatidylserine Electrochemical detection method Microscopy Confocal medicine.diagnostic_test Chemistry Biological techniques Electrochemical Techniques Flow Cytometry 3. Good health Cell biology Outer membrane Quantum dot Microscopy Electron Scanning Protein Binding |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2040-3372 |
| Popis: | There is a great demand to develop novel techniques that allow useful and complete monitoring of apoptosis, which is a key factor of several diseases and a target for drug development. Here, we present the use of a novel dual electrochemical/optical label for the detection and study of apoptosis. We combined the specificity of Annexin-V for phosphatidylserine, a phospholipid expressed in the outer membrane of apoptotic cells, with the optical and electrochemical properties of quantum dots to create a more efficient label. Using this conjugate we addressed three important issues: (i) we made the labeling of apoptotic cells faster (30 min) and easier; (ii) we fully characterized the samples by common cell biological techniques (confocal laser scanning microscopy, scanning electron microscopy and flow cytometry); and (III) we developed a fast, cheap and quantitative electrochemical detection method for apoptotic cells with results in full agreement with those obtained by flow cytometry. The authors thank MICINN (project MAT2011-25870 and TEC2011-29140-C03-03), SMS (EU FP7 project) and the Generalitat de Catalunya (project 2014SGR-524) for supporting this project. |
| Databáze: | OpenAIRE |
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