Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization
| Autor: | K. Larkin, S J Darnton, J C Rockett, H R Matthews, A G Morris |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Esophageal Neoplasms Transplantation Heterologous Mice Nude Malignancy Interferon-gamma Mice Antigens Neoplasm HLA Antigens Transforming Growth Factor beta Genetic variation medicine Carcinoma Tumor Cells Cultured Animals Humans biology Karyotype Transforming growth factor beta medicine.disease Intercellular Adhesion Molecule-1 Phenotype Squamous carcinoma Oncology Cell culture Karyotyping Antigens Surface biology.protein Cancer research Neoplasm Transplantation Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones derived from them. Karyotype analysis indicates genetic variation among the clones. HLA-A, -B and -C is expressed constitutively, but not HLA-DR. ICAM-1-expressing phenotypes may have arisen during adaptation to long-term culture. All lines are capable of response to interferon-gamma (IFN-gamma) and all produce transforming growth factor beta 1 (TGF-beta 1). Two lines are resistant to the inhibitory growth effects of the latter, possibly contributing to malignancy. It is anticipated that these lines, originating from histologically different carcinomas, will provide a valuable, continuous resource for the investigation and treatment of these aggressive tumours. Images Figure 1 Figure 3 |
| Databáze: | OpenAIRE |
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