The role of insulin in the modulation of glucagon-dependent control of phenylalanine hydroxylation in isolated liver cells
| Autor: | Christopher I. Pogson, Michael Fisher, Alan J. Dickson |
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| Rok vydání: | 1987 |
| Předmět: |
Male
medicine.medical_specialty Phenylalanine hydroxylase Phenylalanine medicine.medical_treatment 8-Bromo Cyclic Adenosine Monophosphate Stimulation Cell Separation In Vitro Techniques Peptide hormone Hydroxylation Biochemistry Glucagon chemistry.chemical_compound Internal medicine medicine Animals Insulin Cyclic GMP Molecular Biology Dose-Response Relationship Drug biology Phenylalanine Hydroxylase Rats Inbred Strains Vanadium Cell Biology Metabolism Rats Endocrinology Liver chemistry biology.protein Vanadates Research Article |
| Zdroj: | Biochemical Journal. 242:655-660 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | The stimulation of phenylalanine hydroxylation in isolated liver cells by sub-maximally effective concentrations of glucagon (less than 0.1 microM) is antagonized by insulin (0.1 nM-0.1 microM). This phenomenon is a consequence of a decrease in the glucagon-stimulated phosphorylation of phenylalanine hydroxylase from liver cells incubated in the presence of insulin. The impact of insulin on the phosphorylation state and activity of the hydroxylase is mimicked by incubation of liver cells in the presence of orthovanadate (10 microM). A series of cyclic AMP and cyclic GMP analogues enhanced phenylalanine hydroxylation: in each case insulin diminished the stimulation of flux. These results are discussed in the light of the characteristics of insulin action on other metabolic processes. |
| Databáze: | OpenAIRE |
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