The role of luminal factors in the recovery of gastric function and behavioral changes after chronicHelicobacter pyloriinfection
| Autor: | Mary H. Perdue, E. Tsuchida, Nafia Al-Mutawaly, Thomas A. Tompkins, Premysl Bercik, Hiromi Sakai, Kenneth Croitoru, Stephen M. Collins, Jun Lu, Xian Xi Huang, Elena F. Verdu |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Helicobacter pylori infection Physiology Spirillaceae Helicobacter Infections Mice Antigen Lactobacillus rhamnosus Physiology (medical) medicine Animals Dyspepsia Antigens Bacterial Mice Inbred BALB C Helicobacter pylori Hepatology Gastric emptying biology Lacticaseibacillus rhamnosus Probiotics Stomach Gastroenterology Feeding Behavior biology.organism_classification Lactobacillus helveticus medicine.anatomical_structure Gastric Emptying Gastritis Chronic Disease Immunology Function (biology) |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 295:G664-G670 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | The role of chronic infections, such as Helicobacter pylori ( Hp), to produce sustained changes in host physiology remains controversial. In this study, we investigate whether the antigenic or bacterial content of the gut, after Hp eradication, influences the changes in gut function induced by chronic Hp infection. Mice were infected with Hp for 4 mo and then treated with antibiotics or placebo for 2 wk. Gastric emptying was measured using videofluoroscopy, feeding behavior using a 24-h feeding system, and intestinal permeability using an isolated jejunal segment arterially perfused with an artificial oxygen carrier, hemoglobin vesicles. Immune responses were assessed by CD3+cell counts and anti- Hp antibodies using ELISA. To determine the role of luminal factors in host physiology posteradication, groups of mice received the probiotics containing Lactobacillus rhamnosus R0011 and L. helveticus R0052 or placebo for 2 wk or crude Hp antigen weekly for 2 mo. Chronic Hp infection was associated with delayed gastric emptying, increased intestinal permeability, and increased gastric CD3+cell counts. Hp-induced altered feeding patterns did not reverse after eradication. Probiotics accelerated the recovery of paracellular permeability and delayed gastric emptying, improved the CD3+cell counts, and normalized altered feeding patterns posteradication. Hp antigen resulted in increased anti- Hp antibodies and increased CD3+cell counts in the stomach and delayed recovery of gastric function. Our results suggest that the bacterial content of the gut, as well as the presence of relevant antigens, influences the rate of recovery of host pathophysiology induced by chronic Hp infection. These changes do not seem to occur in association with modulation of intestinal permeability. |
| Databáze: | OpenAIRE |
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