Reduction of all-trans-retinoic acid–induced teratogenesis in the rat by glycine administration

Autor: Eduardo Madrigal-Bujaidar, Germán Chamorro-Cevallos, Carvajal-Sandoval G, Pedro Zamudio-Cortes, Elías Parra-Hernández, Alba Martínez-Angoa
Rok vydání: 2006
Předmět:
Zdroj: Birth Defects Research Part A: Clinical and Molecular Teratology. 76:731-738
ISSN: 1542-0760
1542-0752
DOI: 10.1002/bdra.20309
Popis: BACKGROUND: Prenatal rat embryo exposure to retinoids induces severe malformations in various organs; the most active and teratogenic metabolite is all-trans-retinoic acid (atRA). The mechanisms of this embryopathy are only partly known. In the present study, the influence of glycine on the teratogenicity of atRA was investigated. METHODS: Embryos from 5 groups of white rats were studied: Group 1 remained untreated; Group 2 received glycine 2% in drinking water ad libitum from the first gestational day (GD 1); Group 3 was administered vehicle (corn oil); Group 4 was treated with atRA (50 mg/kg of body weight) injected (IP); and Group 5 was treated with atRA (50 mg/kg of body weight IP) plus glycine 2% in drinking water ad libitum from GD 1. atRA was administrated daily from GD 8–10. Dams were killed on the 21st day of pregnancy, and their fetuses were examined to detect external, visceral, and skeletal malformations. RESULTS: The results show that the atRA-administered dose is not toxic for the dams, and that although fetal death was not observed, it produced abnormalities in the fetuses. Glycine reduced atRA-induced teratogenic effects (external and skeletal defects). CONCLUSIONS: The results indicate that glycine effectively reduces the teratogenic effects of atRA. Thus, glycine might be useful for the prevention of vitamin A teratogenicity. Birth Defects Research (Part A), 2006. © 2006 Wiley-Liss, Inc.
Databáze: OpenAIRE
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