Improving drought-, salinity-, and heat-tolerance in transgenic plants by co-overexpressing Arabidopsis vacuolar pyrophosphatase gene AVP1 and Larrea Rubisco activase gene RCA
| Autor: | Jennifer L. Smith, Li Sun, Hong Zhang, Guoxin Shen, Paxton Payton, Inosha Wijewardene, Xunlu Zhu, Neelam Mishra |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Plant Science Genetically modified crops Photosynthesis 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Genetics Plant Proteins Abiotic component biology Dehydration Abiotic stress Arabidopsis Proteins fungi RuBisCO food and beverages Salt-Tolerant Plants General Medicine biology.organism_classification Plants Genetically Modified Larrea Salinity Horticulture Inorganic Pyrophosphatase 030104 developmental biology biology.protein Agronomy and Crop Science Heat-Shock Response 010606 plant biology & botany |
| Zdroj: | Plant science : an international journal of experimental plant biology. 296 |
| ISSN: | 1873-2259 |
| Popis: | The severity and frequency of many abiotic stresses such as drought, salinity and heat, cause substantial crop losses worldwide, which poses a serious challenge in food security. To increase crop production, new approaches are needed. Previous research has shown that overexpression of the tonoplast H+ pyrophosphatase gene AVP1 leads to improved drought and salt tolerance in transgenic plants. Other research showed that overexpression of thermotolerant ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene could maintain photosynthesis at higher temperatures, which contributes to higher heat tolerance in transgenic plants. In nature, abiotic stresses rarely come alone, instead these stresses often occur in various combinations. Therefore, it is desirable to make crops more tolerant to multiple stresses, which will likely lead to higher crop yield under various stress conditions. It is shown here that co-overexpression of the Arabidopsis gene AVP1 and the Larrea Rubisco activase gene RCA significantly increases drought, salinity and heat tolerance, resulting in higher biomass and seed yield than wild-type plants. AVP1/RCA co-overexpressing plants are as more drought- and salt-tolerant as AVP1-overexpressing plants, and as more heat-tolerant as RCA-overexpressing plants. More importantly, they produce higher seed yields than AVP1-overexpressing, RCA-overexpressing, and wild-type plants under combined drought and heat conditions. |
| Databáze: | OpenAIRE |
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