Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil
| Autor: | Leandro Parussolo, Fernanda Danielle Melo, Ricardo Antonio Pilegi Sfaciotte, Karine Andrezza Dalmina, Sandra Maria Ferraz, Ubirajara Maciel da Costa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Serotype Science 030106 microbiology Virulence Biology medicine.disease_cause Microbiology 03 medical and health sciences 0404 agricultural biotechnology Listeria monocytogenes Cheese multidrug resistance medicine Humans Listeriosis Agar diffusion test Gene Multidisciplinary dairy products raw milk virulence genes 04 agricultural and veterinary sciences Raw milk Antimicrobial 040401 food science Multiple drug resistance Brazil |
| Zdroj: | Anais da Academia Brasileira de Ciências, Vol 93, Iss 3 (2021) Anais da Academia Brasileira de Ciências v.93 n.3 2021 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC |
| ISSN: | 1678-2690 |
| Popis: | Listeria monocytogenes is an opportunistic pathogen that causes listeriosis, a foodborne disease with low incidence but with high mortality rate in humans. This microorganism has been recovered from several dairy products, especially those produced with raw milk. The objective of this work was to investigate the presence of virulence genes, and also to define the antimicrobial susceptibility profile of L. monocytogenes isolates recovered from serrano artisanal cheese produced in Southern region of Brazil. Nine strains of L. monocytogenes (serotypes 1/2b and 4b) were evaluated through PCR to detect the presence of the virulence genes hly, inlA, inlC, inlJ, actA, plcB and iap, while antimicrobial susceptibility profile was determined via disk diffusion method. All strains exhibited the presence of the genes hly and plcB, whereas the other genes (iap, actA, inlA, inlC and inlJ) were only detected in eight strains. We verified that all strains were resistant to at least one antimicrobial agent and three of them showed multidrug resistance. These findings demonstrated the serrano artisanal cheese offers risks to consumers’ health and point to a need of adaptations and monitoring of manufacturing process of this food, in order to prevent the dissemination of L. monocytogenes. |
| Databáze: | OpenAIRE |
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