UV Induces GADD45 in a p53-Dependent and -Independent Manner in Human Keratinocytes
| Autor: | Duane A. Leedel, Le Luong, Eugene G. Chomey, Tomoko Maeda, Victor A. Tron, Adrian B. Sim, Prescillia P. S. Chua |
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| Rok vydání: | 2003 |
| Předmět: |
Keratinocytes
Ultraviolet Rays business.industry Blotting Western Intracellular Signaling Peptides and Proteins Proteins Dermatology Molecular biology Up-Regulation 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation 030220 oncology & carcinogenesis Humans Medicine Surgery RNA Messenger Tumor Suppressor Protein p53 business Ultraviolet radiation |
| Zdroj: | Journal of Cutaneous Medicine and Surgery. 7:119-123 |
| ISSN: | 1615-7109 1203-4754 |
| DOI: | 10.1177/120347540300700204 |
| Popis: | Background:GADD45 is a multifunctional protein involved in DNA repair and in cell cycle checkpoint control. p53 plays an important role in regulating DNA repair and in response to UVB in keratinocytes.Objective:GADD45 and p53 expression was examined and compared at the mRNA and protein level after exposure to UV irradiation.Methods:Human keratinocytes were exposed to increasing doses of UVB, and an RNA protection assay and a Western blot analysis were performed.Results:The RNase protection assays using human keratinocytes showed that GADD45 mRNA increases after 4 h and remains elevated for 24 h in cells irradiated at 100, 300, or 600 J/m2UVB. The level of GADD45 protein increases after 8 h and remains elevated for 48 h, with maximal induction at 300 J/m2. p53 mRNA did not rise in concert with GADD45 at any dose used, and p53 protein was not up-regulated at the lower dose of 100 J/m2.Conclusion:GADD45 is regulated in both a p53-dependent and a p53-independent manner in keratinocytes after UV exposure. |
| Databáze: | OpenAIRE |
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