A Comparison of Two Ovine Lumbar Intervertebral Disc Injury Models for the Evaluation and Development of Novel Regenerative Therapies
| Autor: | Tanya Badal, Graham Jenkin, Chris D. Daly, Justin J. Cooper-White, Peter Ghosh, Tony Goldschlager, Ronald Shimmon, Idrees Sher, Ronil V. Chandra, David Oehme |
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| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty animal structures medicine.medical_treatment Lumbar discectomy 03 medical and health sciences 0302 clinical medicine Animal model discectomy Discectomy medicine Orthopedics and Sports Medicine Lumbar intervertebral disc 030203 arthritis & rheumatology business.industry animal model Intervertebral disc Original Articles Surgery medicine.anatomical_structure regeneration intervertebral disc Neurology (clinical) business 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists Large animal |
| Zdroj: | Global Spine Journal |
| ISSN: | 2192-5682 |
| Popis: | Study Design: Large animal research. Objective: Lumbar discectomy is the most commonly performed spinal surgical procedure. We investigated 2 large animal models of lumbar discectomy in order to study the regenerative capacity of mesenchymal stem cells following disc injury. Methods: Twelve adult ewes underwent baseline 3-T magnetic resonance imaging (MRI) followed by lumbar intervertebral disc injury by either drill bit (n = 6) or annulotomy and partial nucleotomy (APN) (n = 6). Necropsies were performed 6 months later. Lumbar spines underwent 3-T and 9.4-T MRI prior to histological, morphological and biochemical analysis. Results: Drill bit-injured (DBI) and APN-injured discs demonstrated increased Pfirrmann grades relative to uninjured controls (P < .005), with no difference between the 2 models. Disc height index loss was greater in the APN group compared with the DBI group (P < .005). Gross morphology injury scores were higher in APN than DBI discs (P < .05) and both were higher than controls (P < .005). Proteoglycan was reduced in the discs of both injury models relative to controls (P < .005), but lower in the APN group (P < .05). Total collagen of the APN group disc regions was higher than DBI and control discs (P < .05). Histology revealed more matrix degeneration, vascular infiltration, and granulation in the APN model. Conclusion: Although both models produced disc degeneration, the APN model better replicated the pathobiology of human discs postdiscectomy. We therefore concluded that the APN model was a more appropriate model for the investigation of the regenerative capacity of mesenchymal stem cells administered postdiscectomy. |
| Databáze: | OpenAIRE |
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