Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria
| Autor: | Anna Maria Timperio, Palmiro Cantatore, Vito Pesce, Luigi Mosconi, Lello Zolla, Maria Nicola Gadaleta, Valentina Capelli, Clara Musicco, Menotti Calvani |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Aging Peroxiredoxin III Biophysics Mitochondria Liver Mitochondrion Protein oxidation medicine.disease_cause Rat liver mitochondria Biochemistry Mass Spectrometry medicine Animals Electrophoresis Gel Two-Dimensional Cysteine Carnitine Polyacrylamide gel electrophoresis Nootropic Agents Gel electrophoresis Mass spectrometry Two-dimensional electrophoresis Chemistry Peroxiredoxins Cell Biology Molecular biology Rats Inbred F344 Rats Molecular Weight Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Acetylcarnitine Oxidation-Reduction Oxidative stress medicine.drug |
| Zdroj: | Biochimica et biophysica acta. Bioenergetics 1787 (2009): 890–896. doi:10.1016/j.bbabio.2009.03.002 info:cnr-pdr/source/autori:Musicco C (a); Capelli V (b); Pesce V (b); Timperio AM (c); Calvani M (d); Mosconi L (d); Zolla L (c); Cantatore P (a,b); Gadaleta MN (a,b)./titolo:Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria/doi:10.1016%2Fj.bbabio.2009.03.002/rivista:Biochimica et biophysica acta. Bioenergetics/anno:2009/pagina_da:890/pagina_a:896/intervallo_pagine:890–896/volume:1787 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2009.03.002 |
| Popis: | Overoxidation and subsequent inactivation of Peroxiredoxin III (PrxIII), a mitochondrial H2O2 scavenging enzyme, have been reported in oxidative stress conditions. No data are available in the literature about the presence of overoxidized forms of PrxIII in aged tissues. Liver mitochondria from 12-month-old rats and 28-month-old rats were here analyzed by two-dimensional gel electrophoresis. A spot corresponding to the native form of PrxIII was present in adult and old rats with the same volume, whereas an additional, more acidic spot, of the same molecular weight of the native form, accumulated only in old rats. The acidic spot was identified, by MALDI-MS analysis, as a form of PrxIII bearing the cysteine of the catalytic site overoxidized to sulphonic acid. This modified PrxIII form corresponds to the irreversibly inactivated enzyme, here reported, for the first time, in aging. Three groups of 28-month-old rats treated with acetyl- l -carnitine were also examined. Reduced accumulation of the overoxidized PrxIII form was found in all ALCAR-treated groups. |
| Databáze: | OpenAIRE |
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