| Popis: |
PDF file - 214K, Supplemental Figure 6: Monocyte-derived DC exhibit a similar level of NYESO-1 cross-presentation compared to pDC. (A) on day0, pDC and monocytes from a single donor were purified. pDC were frozen and monocytes cultured with IL-4 and GM-CSF. On day 5, pDC were thawed. pDC and monocyte-dervied DC were cultured alone or with UV-irradiated M18 (M18UV) or MV-infected M18 (M18MV) for 18hrs. On day6, DC were cultured with the NYESO-1 specific CD8+ T cell clone during 6hrs in presence of brefeldinA. Cells were then stained with anti-CD8+ antibody, fixed, permeabilized and stained with IFN-g specific antibody. IFN-g secretion by the the T cell clone was analyzed by flow cytometry. We conclude that level of cross-presentation was somewhat similar between the two cell types. It was a little bit weaker for pDC, but these cells have been frozen, whereas Monocyte-derived DC were not. (B) scatter plot representation of three cross-presentation experiments performed with monocyte-derived DC. Error bars represent standard deviation. |
