Quantitative expression of lysophosphatidic acid receptor 3 gene in porcine endometrium during the periimplantation period and estrous cycle
| Autor: | Iwona Bogacka, M. Bogacki, Marta Wasielak, Katarzyna Kamińska, Michał Blitek |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
DNA Complementary Swine Physiology Biology Endometrium Polymerase Chain Reaction Biochemistry chemistry.chemical_compound Estrus Pregnancy Sequence Homology Nucleic Acid Internal medicine Lysophosphatidic acid Reproductive biology Gene expression medicine Animals Humans Embryo Implantation RNA Messenger Receptors Lysophosphatidic Acid DNA Primers Pharmacology Estrous cycle Base Sequence Uterine horns Embryo Cell Biology Endocrinology medicine.anatomical_structure chemistry Female Lysophosphatidic Acid Receptor 3 |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 85:26-32 |
| ISSN: | 1098-8823 |
| DOI: | 10.1016/j.prostaglandins.2007.10.001 |
| Popis: | Lysophosphatidic acid (LPA) belongs to the group of lipid messengers, which act via lysophosphatidic acid receptor 3 coupled to G-proteins. The participation of LPA3 in reproductive biology was revealed in mice and has not been studied in gilts. The present study was performed to evaluate the gene expression of LPA3 by a quantitative real-time PCR technique in the endometrium during different stages of pregnancy (days 6–30) and corresponding days of the estrous cycle (days 2–20) as well as in periimplantation period in pigs with surgically detached uterine horns. Based on the most conserved segments of human and rodent LPA3 we obtained a product containing 619 bp (GenBank: EF137953 ), which exhibited high homology with human and rodents sequences. The highest transcript level was noted on days 10–12 of gestation in comparison to remaining periods and during pregnancy on days: 6–7, 8–9, 10–12 and 13–14 in comparison with the corresponding days of the estrous cycle. Higher mRNA level was noted in the horn containing embryos compared to the contralateral horn, where embryos did not develop. The results imply the important role of receptor LPA3 during early pregnancy. |
| Databáze: | OpenAIRE |
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