Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Autor:	Natsuki, Kawai, Yuki, Hirakawa, Shinya, Matsumoto, Akihiro, Itai, Kayoko, Matsunaga, Hiroshi, Narita, Keiko, Momma
Rok vydání:	2022
Předmět:	Prunus persica Organic Chemistry Reverse Transcription General Medicine Allergens Antigens Plant Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry Actins Gibberellins Analytical Chemistry Fruit Molecular Biology Plant Proteins Biotechnology
Zdroj:	Bioscience, Biotechnology, and Biochemistry. 86:1459-1461
ISSN:	1347-6947
DOI:	10.1093/bbb/zbac123
Popis:	Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55514b7133708cfc407ca5483e753ce5 https://doi.org/10.1093/bbb/zbac123 Zobrazit plný text záznamu