Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds
| Autor: | Xiaochu Ma, Päivi Tammela, Polina Ilina, Herman O. Sintim |
|---|---|
| Přispěvatelé: | Bioactivity Screening Group, Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) High-throughput screening Archaeal Proteins 030106 microbiology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Assay miniaturization Pentanones High throughput screening Drug Discovery Drug Resistance Bacterial medicine Escherichia coli Molecular Biology 1183 Plant biology microbiology virology Quorum sensing inhibitors biology Bacteria Escherichia coli Proteins AI-2 Bioreporter strain Quorum Sensing Pathogenicity biology.organism_classification beta-Galactosidase Autoinducer-2 AUTOINDUCER-2 Quorum sensing 030104 developmental biology chemistry PERSPECTIVES 317 Pharmacy 1182 Biochemistry cell and molecular biology Biological Assay Bioreporter Whole cell Carrier Proteins INHIBITORS ART Signal Transduction |
| Popis: | The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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