Skin-draining lymph nodes contain dermis-derived CD103(-) dendritic cells that constitutively produce retinoic acid and induce Foxp3(+) regulatory T cells
| Autor: | Sandrine Henri, Lena Alexopoulou, Elisabeth Devilard, Bernard Malissen, Karine Crozat, Béatrice de Bovis, Marc Dalod, Samira Tamoutounour, Martin Guilliams, Pierre Grenot |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Technologies avancées pour le génôme et la clinique (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Retinoic acid
MESH: Lymph Nodes Lymphocyte Activation Biochemistry T-Lymphocytes Regulatory MESH: Mice Knockout chemistry.chemical_compound Mice 0302 clinical medicine MESH: Animals Intestinal Mucosa Lymph node Lung MESH: Antigens CD Cells Cultured Skin Mice Knockout MESH: Langerhans Cells 0303 health sciences medicine.diagnostic_test FOXP3 Forkhead Transcription Factors hemic and immune systems Hematology Cell biology Intestines Isoenzymes medicine.anatomical_structure MESH: Isoenzymes [SDV.IMM]Life Sciences [q-bio]/Immunology Integrin alpha Chains MESH: Cells Cultured MESH: Intestines Immunology Tretinoin chemical and pharmacologic phenomena Biology Aldehyde Dehydrogenase 1 Family Flow cytometry 03 medical and health sciences Antigen MESH: Skin Antigens CD MESH: Mice Inbred C57BL MESH: Forkhead Transcription Factors medicine Animals MESH: Lung Antigen-presenting cell MESH: Lymphocyte Activation MESH: Mice 030304 developmental biology MESH: Tretinoin MESH: T-Lymphocytes Regulatory MESH: Integrin alpha Chains Retinal Dehydrogenase Cell Biology T lymphocyte Dendritic cell Aldehyde Dehydrogenase Mice Inbred C57BL MESH: Aldehyde Dehydrogenase chemistry Langerhans Cells NIH 3T3 Cells Lymph Nodes 030215 immunology MESH: NIH 3T3 Cells |
| Zdroj: | Blood Blood, American Society of Hematology, 2010, 115 (10), pp.1958-68. ⟨10.1182/blood-2009-09-245274⟩ Blood, 2010, 115 (10), pp.1958-68. ⟨10.1182/blood-2009-09-245274⟩ Blood; Vol 115 |
| ISSN: | 0006-4971 1528-0020 |
| DOI: | 10.1182/blood-2009-09-245274⟩ |
| Popis: | Small intestinal CD103+ dendritic cells (DCs) have the selective ability to promote de novo generation of regulatory T cells via the production of retinoic acid (RA). Considering that aldehyde dehydrogenase (ALDH) activity controls the production of RA, we used a flow cytometry–based assay to measure ALDH activity at the single-cell level and to perform a comprehensive analysis of the RA-producing DC populations present in lymphoid and nonlymphoid mouse tissues. RA-producing DCs were primarily of the tissue-derived, migratory DC subtype and can be readily found in the skin and in the lungs as well as in their corresponding draining lymph nodes. The RA-producing skin-derived DCs were capable of triggering the generation of regulatory T cells, a finding demonstrating that the presence of RA-producing, tolerogenic DCs is not restricted to the intestinal tract as previously thought. Unexpectedly, the production of RA by skin DCs was restricted to CD103− DCs, indicating that CD103 expression does not constitute a “universal” marker for RA-producing mouse DCs. Finally, Toll-like receptor (TLR) triggering or the presence of a commensal microflora was not essential for the induction of ALDH activity in the discrete ALDH+ DC subsets that characterize tissues constituting environmental interfaces. |
| Databáze: | OpenAIRE |
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