The value of microscopic-observation drug susceptibility assay in the diagnosis of tuberculosis and detection of multidrug resistance
| Autor: | Deniz Sertel Selale, Meltem Uzun |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Tuberculosis 030106 microbiology Microbial Sensitivity Tests Pathology and Forensic Medicine Microbiology Mycobacterium tuberculosis Multidrug Resistant Tuberculosis 03 medical and health sciences 0302 clinical medicine Drug Resistance Multiple Bacterial Isoniazid Immunology and Allergy Medicine Humans 030212 general & internal medicine Rifampicin Microscopy biology business.industry Sputum General Medicine Drug susceptibility medicine.disease biology.organism_classification Multiple drug resistance Mycobacterium tuberculosis complex medicine.symptom business Mycobacterium Tuberculosis medicine.drug |
| Zdroj: | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 126(1) |
| ISSN: | 1600-0463 |
| Popis: | Inexpensive, rapid, and reliable tests for detecting the presence and drug susceptibility of Mycobacterium tuberculosis complex (MTBC) are urgently needed to control the transmission of tuberculosis. In this study, we aimed to assess the accuracy and speed of the microscopic-observation drug susceptibility (MODS) assay in the identification of MTBC and detection of multidrug resistance. Sputum samples from patients suspected to have tuberculosis were simultaneously tested with MODS and conventional culture [Löwenstein-Jensen (LJ) culture, BACTEC MGIT™ 960 (MGIT) system], and drug susceptibility testing (MGIT system) methods. A total of 331 sputum samples were analyzed. Sensitivity and specificity of MODS assay for detection of MTBC strains were 96% and 98.8%, respectively. MODS assay detected multidrug resistant MTBC isolates with 92.3% sensitivity and 96.6% specificity. Median time to culture positivity was similar for MGIT (8 days) and MODS culture (8 days), but was significantly longer with LJ culture (20 days) (p |
| Databáze: | OpenAIRE |
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