Functional analysis of the human lecithin cholesterol acyl transferase gene promoter
| Autor: | Roberto Taramelli, Sergio Ottolenghi, Germana Meroni, Marco Pontoglio, Nicoletta Malgaretti |
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| Přispěvatelé: | Meroni, Germana, Malgaretti, Nicoletta, Pontoglio, Marco, Ottolenghi, Sergio, Taramelli, Roberto |
| Rok vydání: | 1991 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Carcinoma Hepatocellular TATA box Molecular Sequence Data Biophysics Biology Transfection Biochemistry Cell Line Phosphatidylcholine-Sterol O-Acyltransferase Transcription (biology) Gene expression Humans Promoter Regions Genetic Site-directed mutagenesis Molecular Biology Gene promoter Base Sequence Liver Neoplasms Promoter Cell Biology Transferase Gene Molecular biology Recombinant Proteins Oligodeoxyribonucleotides Regulatory sequence Mutagenesis Site-Directed Lecithin cholesterol acyl transferase gene promoter Lecithin cholesterol acyl transferase gene HeLa Cells Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 180:1469-1475 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(05)81361-0 |
| Popis: | Summary In this report cis-acting sequences that direct transcription of the lecithin choleterol acyl transferase (LCAT) gene were identified. To assay the promoter activity, fragments from the 5′ flanking region were fused upstream to the cloramphenicol acetyl transferase gene and transfected into Hep3B and HeLa cells. The gene sequences were active in both cell lines. A minimal promoter comprising only 71 bp is still fully active and contains a TATA box, a LFAI motif and two Sp1 binding sites. The activity of the promoter was entirely dependent on the Spl sites. |
| Databáze: | OpenAIRE |
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