Glucose detection at bare and sputtered platinum electrodes coated with polypyrrole and glucose oxidase
| Autor: | C.G.J. Koopal, P.J.H.J. van Os, Auke Bult, W.P. van Bennekom |
|---|---|
| Přispěvatelé: | Centraal Instituut voor Voedingsonderzoek TNO |
| Rok vydání: | 1996 |
| Předmět: |
GOx
Multichannel electrochemical detection Inorganic chemistry chemistry.chemical_element Polypyrrole Biochemistry Analytical Chemistry chemistry.chemical_compound Electron transfer Environmental Chemistry Selectivity Glucose oxidase (Spudered)platinum Spectroscopy biology FAD Ascorbic acid Flavine adenine dinucIeotide chemistry Polymerization (Poly-)pyrrole (Direct) electron-transfer Electrode biology.protein Platinum |
| Zdroj: | Analytica Chimica Acta, 3, 335, 209-216 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/s0003-2670(96)00327-3 |
| Popis: | Recently a glucose sensor has been developed, which is easily prepared, has a good stability and high glucose response, and is interference free for ascorbic acid. The sensor was made by galvanostatic polymerization of 0.3 M pyrrole in a HEPES buffer pH 7.0 with 200 U ml-1 glucose oxidase (GOx) using a current of 1 μA. In this study, bare and sputtered platinum electrodes, coated with polypyrrole in the presence of GOx and/or flavine adenine dinucleotide (FAD), are compared. FAD in excess in the polymerization or sample solution did not increase the glucose response. The selectivity with respect to other mono and oligosaccharides was good. The mechanism of electron transfer was studied. From measurements at low potentials and at anaerobic conditions there is a strong indication that the glucose sensor is a combination of indirect and direct electron-transfer (first and third generation, respectively). Chemicals/CAS: glucose oxidase, 9001-37-0; platinum, 7440-06-4; polypyrrole, 30604-81-0 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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