De novo assembly and annotation of Hyalomma dromedarii tick (Acari: Ixodidae) sialotranscriptome with regard to gender differences in gene expression

Autor: Ursula Castro de Oliveira, Inácio L.M. Junqueira-de-Azevedo, Cherif Ben Hamda, Ali Bouattour, Kais Ghedira, Flavio Lichtenstein, Fernanda Faria, Ana Marisa Chudzinski-Tavassi, Chaima Bensaoud, Youmna M’ghirbi, Milton Y. Nishiyama
Přispěvatelé: Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire (LR11IPT03), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), Instituto Butantan [São Paulo], Laboratoire de Bioinformatique, biomathématiques, biostatistiques (BIMS) (LR11IPT09), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Tunis El Manar (UTM), This work was conducted with financial support from the Collaborative internal project PCI-05/2012-2015 IPT. The authors (Milton Yutaka Nishiyama Jr., Flavio Lichtenstein, Ursula Castro de Oliveira, Fernanda Faria, Inácio Loiola Meirelles Junqueira-de-Azevedo and Ana Marisa Chudzinski-Tavassi) received financial support from CAPES (Auxpe-Toxinologia 1207/2011) and FAPESP (2013/07467-1)., We would like to thank Dr Deborah Glassman for her English corrections and constructive comments on the manuscript.
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Saliva
MESH: Ixodidae/chemistry
[SDV]Life Sciences [q-bio]
Hyalomma dromedarii
Salivary Glands
0302 clinical medicine
Camels
Acari
MESH: Animals
MESH: Phylogeny
Phylogeny
MESH: Sequence Analysis
DNA/veterinary
MESH: Gene Expression Profiling/veterinary
Genetics
MESH: Transcriptome
biology
Sialotranscriptome
High-Throughput Nucleotide Sequencing
MESH: High-Throughput Nucleotide Sequencing/veterinary
Infectious Diseases
Gene enrichment
MESH: Camelus/parasitology
Sialome
Female
MESH: Tunisia
Ixodidae
Hyalomma dromedarii tick
Camelus
Tunisia
MESH: Salivary Glands/chemistry
030231 tropical medicine
MESH: Molecular Sequence Annotation
Tick
MESH: Host-Parasite Interactions
Arthropod Proteins
Host-Parasite Interactions
lcsh:Infectious and parasitic diseases
03 medical and health sciences
MESH: Gene Library
MESH: Arthropod Proteins/genetics
Animals
lcsh:RC109-216
Illumina dye sequencing
Gene Library
Gene Expression Profiling
Research
MESH: Ixodidae/genetics
Molecular Sequence Annotation
Sequence Analysis
DNA
biology.organism_classification
MESH: Male
030104 developmental biology
Parasitology
Gene expression
MESH: Saliva/chemistry
Hyalomma
Transcriptome
MESH: Female
Zdroj: Parasites and Vectors
Parasites and Vectors, BioMed Central, 2018, 11 (1), ⟨10.1186/s13071-018-2874-9⟩
Parasites & Vectors, Vol 11, Iss 1, Pp 1-16 (2018)
Parasites & Vectors
ISSN: 1756-3305
Popis: Background Hard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activities carried out by tick saliva molecules warrant their pharmacological investigation. The Hyalomma dromedarii Koch, 1844 tick is a common parasite of camels and probably the best adapted to deserts of all hard ticks. Like other hard ticks, the salivary glands of this tick may provide a rich source of many compounds whose biological activities interact directly with host system pathways. Female H. dromedarii ticks feed longer than males, thereby taking in more blood. To investigate the differences in feeding behavior as reflected in salivary compounds, we performed de novo assembly and annotation of H. dromedarii sialotranscriptome paying particular attention to variations in gender gene expression. Results The quality-filtered Illumina sequencing reads deriving from a cDNA library of salivary glands led to the assembly of 15,342 transcripts. We deduced that the secreted proteins included: metalloproteases, glycine-rich proteins, mucins, anticoagulants of the mandanin family and lipocalins, among others. Expression analysis revealed differences in the expression of transcripts between male and female H. dromedarii that might explain the blood-feeding strategies employed by both genders. Conclusions The annotated sialome of H. dromedarii helps understand the interaction of tick-host molecules during blood-feeding and can lead to the discovery of new pharmacologically active proteins of ticks of the genus Hyalomma. Electronic supplementary material The online version of this article (10.1186/s13071-018-2874-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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