Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton
| Autor: | Diane Egger-Adam, Maxime G. Blanchard, Anne Marie Lüchtenborg, Stephan Kellenberger, Vladimir L. Katanaev, Chen Lin, Gonzalo P. Solis, Alexey Koval |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Ankyrins
Frizzled animal structures Neurite Neuromuscular Junction Wnt1 Protein Biology Ankyrin GTP-Binding Protein alpha Subunits Gi-Go Microtubules Cell Line 03 medical and health sciences Wnt Mice 0302 clinical medicine Microtubule ddc:570 Heterotrimeric G protein Neurites Animals Drosophila Proteins Molecular Biology Research Articles Conserved Sequence Drosophila Neuromuscular junction Wnt Frizzled G protein Ankyrin Microtubules 030304 developmental biology chemistry.chemical_classification Mammals Neurons 0303 health sciences fungi Wnt signaling pathway G protein Frizzled Receptors Cell biology Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] Drosophila melanogaster chemistry Synapses Drosophila Glutamatergic synapse 030217 neurology & neurosurgery Drosophila Protein Developmental Biology Protein Binding Signal Transduction |
| Zdroj: | Development (Cambridge, England) Development, 141, 3399-409 Development, vol. 141, no. 17, pp. 3399-3409 Development Development, 141, 17, pp. 3399-409 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Item does not contain fulltext Drosophila neuromuscular junctions (NMJs) represent a powerful model system with which to study glutamatergic synapse formation and remodeling. Several proteins have been implicated in these processes, including components of canonical Wingless (Drosophila Wnt1) signaling and the giant isoforms of the membrane-cytoskeleton linker Ankyrin 2, but possible interconnections and cooperation between these proteins were unknown. Here, we demonstrate that the heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse. We identify Ankyrin 2 as a target of Go signaling required for NMJ formation. Moreover, the Go-ankyrin interaction is conserved in the mammalian neurite outgrowth pathway. Without ankyrins, a major switch in the Go-induced neuronal cytoskeleton program is observed, from microtubule-dependent neurite outgrowth to actin-dependent lamellopodial induction. These findings describe a novel mechanism regulating the microtubule cytoskeleton in the nervous system. Our work in Drosophila and mammalian cells suggests that this mechanism might be generally applicable in nervous system development and function. 01 september 2014 |
| Databáze: | OpenAIRE |
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