Monoclonal antibody against TXB2: Its use in solid and liquid phase radirmmunoassays
| Autor: | D S Linthicum, C L Lane, E R Hall |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Physiology
medicine.drug_class Radioimmunoassay Liquid phase Alpha (ethology) Monoclonal antibody Biochemistry Mice chemistry.chemical_compound Endocrinology Phase (matter) medicine Animals Humans Mice Inbred BALB C Chromatography biology medicine.diagnostic_test Chemistry Antibodies Monoclonal Thromboxane B2 Immunoassay biology.protein lipids (amino acids peptides and proteins) Antibody circulatory and respiratory physiology |
| Zdroj: | Prostaglandins, Leukotrienes and Medicine. 30:103-109 |
| ISSN: | 0262-1746 |
| DOI: | 10.1016/0262-1746(87)90140-5 |
| Popis: | Monoclonal antibodies to thromboxane B2 (TXB2) have been produced and characterized. Both liquid and solid phase radioimmunoassays have been developed using one of these monoclonal antibodies. The two assays gave similar results when used to quantitate TXB2 in 11 serum samples; however the solid phase assay was more sensitive than the liquid phase assay (i.e., 63 pg/ml vs 19 pg/ml) at a B/BO = 90%). Despite a difference in the sensitivity of the two assay systems, the cross-reactivity of the monoclonal antibody for PGD2, PGE2, PGF2 alpha and 6 keto-PGF1 alpha was the same. |
| Databáze: | OpenAIRE |
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