Analysis of chromosome karyotype and genome size in echiuran Urechis unicinctus Drasche, 1880 (Polychaeta, Urechidae)
| Autor: | Zhifeng Zhang, Li Lu, Danwen Liu, Zhenkui Qin, Xueyu Li, Qing Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Insecta
Archiannelida Annelida Plant Science Carbotriplurida chemistry.chemical_compound Urechis unicinctus Molecular Cytogenetics Bilateria Genetics Pterygota biology Cephalornis Karyotype Circumscriptional names Boltonocostidae Phylogenesis Circumscriptional name genome size Coelenterata Schizophora Research Article Biotechnology medicine.medical_specialty Arthropoda lcsh:QH426-470 Origoasilidae Nephrozoa Protostomia Basal China Seas Circumscriptional names of the taxon under Muscomorpha Tachinidae Hennigmatidae Panorpida medicine Animalia Echiuroidea Eumetabola Mediosetiger Genome size Oestroidea Urechidae Calyptratae Diptera flow cytometry Cytogenetics Chromosome Polychaeta Strashila incredibilis biology.organism_classification karyotype lcsh:Genetics chemistry Notchia Ecdysozoa Antliophora Coelom Animal Science and Zoology DNA |
| Zdroj: | Comparative Cytogenetics, Vol 13, Iss 1, Pp 75-85 (2019) Comparative Cytogenetics 13(1): 75-85 Comparative Cytogenetics |
| ISSN: | 1993-078X 1993-0771 |
| DOI: | 10.3897/compcytogen.v13i1.31448 |
| Popis: | Karyotype and genome size are two primary cytogenetic characteristics of species, which are of great significance to the study of cytogenetics, taxonomy, phylogenesis, evolution as well as molecular biology. However, this basic cytogenetic information in echiurans is lacking. Therefore, we analyzed characteristics of karyotype and genome size in the echiuran worm Urechisunicinctus Drasche, 1880. In this study, coelomic cells of U.unicinctus were used for analyzing the genome size by a flow cytometry with chicken erythrocytes as DNA standard, and the 2C DNA content was determined to be 1.85 pg, which was corresponded to the genome size of 904.58 Mbp approximately. Furthermore, trochophores of U.unicinctus were dissociated and cells were utilized for preparing the chromosomes stained with DAPI, and the karyotype was determined as 2n = 30 (10m + 6sm + 6st + 8t), FN=52. Our data provided the basic cytogenetic information of U.unicinctus, which could be utilized in taxonomic study and whole-genome sequencing in future. |
| Databáze: | OpenAIRE |
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