Detection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method
| Autor: | Özgün Öykü Özdemir, Ahmet Güner, Nihat Telli, Ferda Soyer Dönmez |
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| Přispěvatelé: | Selçuk Üniversitesi, TR114400, TR115395, Soyer Dönmez, Ferda, Özdemir, Özgün Öykü, Izmir Institute of Technology. Molecular Biology and Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Veterinary Pickled white cheese Antibiotic resistance pickled white cheese 030106 microbiology White cheese PFGE Biology Contamination medicine.disease_cause Listeria monocytogenes serotyping 03 medical and health sciences PCR Pulsed-field gel electrophoresis medicine Food science Serotyping Genotyping |
| Popis: | WOS: 000387808500016 This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates (3.53%) obtained from raw milk, wall/ground, and press cases were identified as L. monocytogenes via the conventional culture method and confirmed by polymerase chain reaction. These isolates were found to belong to serotype 4b. According to antibiotic resistance testing against 10 antibiotics (ampicillin, gentamicin, erythromycin, tetracycline, chloramphenicol, cefalotin, streptomycin, vancomycin, penicillin, and sulfamethoxazole/trimethoprim), it was determined that isolates from raw milk and press cases were resistant to erythromycin. PPGE band patterns of the isolates displayed indistinguishable with AscI and 80%-94% homology with ApaI. The isolates were observed to display high homology to ATCC (13932) and lower homology to EGD SLCC (5835) obtained by both enzymes. Selcuk University Scientific Research Projects Coordination UnitSelcuk University [09102044] This manuscript was summarized from the PhD thesis of the first author and was supported by the Selcuk University Scientific Research Projects Coordination Unit with the project number of 09102044. A part of this study was presented at the 2nd International Food Technology Congress in Kusadasi, Turkey. |
| Databáze: | OpenAIRE |
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