Role of claudin interactions in airway tight junctional permeability
| Autor: | Carolyn B. Coyne, Larry G. Johnson, Todd M. Gambling, Richard C. Boucher, Johnny L. Carson |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Cell Membrane Permeability endocrine system diseases Physiology Bronchi Respiratory Mucosa Biology Transfection urologic and male genital diseases digestive system Cell junction Tight Junctions Mice Physiology (medical) Claudin-1 medicine Animals Claudin-3 Humans Claudin-5 Claudin Tight junction Membrane Proteins 3T3 Cells Cell Biology Recombinant Proteins digestive system diseases Transmembrane protein Cell biology Junctional permeability Airway tissues |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 285:L1166-L1178 |
| ISSN: | 1522-1504 1040-0605 |
| Popis: | Airway epithelial tight junctions (TJs) serve to separate the external and internal environments of the lung. However, the members of the claudin family that mediate this function have not been fully delineated. We characterized the claudin expression in normal airways removed from human donors during lung transplantation and determined the contribution of each claudin to airway barrier function. Stable cell lines in NIH/3T3 and human airway (IB3.1) cells were constructed expressing the claudin components found in the human airway, claudin-1, -3, or -5. The effects of claudin expression on transepithelial resistance, permeability coefficients, and claudin-claudin interactions were assessed. Claudin-1 and -3 decreased solute permeability, whereas claudin-5 increased permeability. We also detected oligomerization of claudin-5 in cell lines and in freshly excised human airways. Coimmunoprecipitation studies revealed heterophilic interactions between claudin species in both cell lines and human airway epithelium. These suggest that airway TJs are regulated by claudinclaudin interactions that confer the selectivity of the junction. |
| Databáze: | OpenAIRE |
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