Activation of caspase-12, an endoplasmic reticulum resident caspase, after permanent focal ischemia in rat
| Autor: | Jennifer L. Zechel, Jorge L. Gamboa, Robert A. Ratcheson, W. David Lust, Warren R. Selman, Graham Mouw |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Ischemia Striatum Endoplasmic Reticulum Brain Ischemia Cortex (anatomy) medicine Animals cardiovascular diseases Rats Wistar Caspase 12 Caspase biology business.industry General Neuroscience Endoplasmic reticulum medicine.disease Rats Enzyme Activation medicine.anatomical_structure Cerebral cortex Caspases biology.protein business Immunostaining |
| Zdroj: | NeuroReport. 14:183-186 |
| ISSN: | 0959-4965 |
| Popis: | The endoplasmic reticulum (ER) is emerging as a contributory component of cell death after ischemia. Since caspase-12 has been localized to the ER and is a novel signal for apoptosis, we examined the message levels and protein expression of caspase-12 after cerebral ischemia in vivo. Animals underwent permanent middle cerebral artery occlusion (MCAO) and were sacri¢ced 24 h after ischemia. Protein analysis revealed a signi¢cant increase in caspase-12 and a corresponding up-regulation of caspase-12 mRNA in the ischemia group compared with that in the sham group. Immunohistochemical analysis revealed diiuse positive immunostaining of caspase-12 throughout the striatum and cerebral cortex in animals that underwent ischemia, with more intense caspase-12 immunostaining in the striatum than in the cortex after ischemia. These results demonstrate that cerebral ischemia initiates an ERbased stress response that results in the transcriptional up-regulation and corresponding increased expression of caspase-12 protein, and may provide a new area for therapeutic intervention to ameliorate outcomes following stroke. NeuroReport 14:183^186 � c 2003 Lippincott Williams & Wilkins. |
| Databáze: | OpenAIRE |
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