Mutation of serum response factor phosphorylation sites and the mechanism by which its DNA-binding activity is increased by casein kinase II
| Autor: | Ron Prywes, J R Manak |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Serum Response Factor
Macromolecular Substances Protein Conformation Molecular Sequence Data Mutant Biology Serine Protein structure Serum response factor Animals Amino Acid Sequence Phosphorylation Protein kinase A Molecular Biology Base Sequence Nuclear Proteins Cell Biology Recombinant Proteins DNA-Binding Proteins Biochemistry Mutagenesis Site-Directed Casein kinase 2 Oligonucleotide Probes Casein kinases Casein Kinases Protein Kinases Research Article Plasmids Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 11:3652-3659 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.11.7.3652 |
| Popis: | Casein kinase II (CKII) phosphorylates the mammalian transcription factor serum response factor (SRF) on a serine residue(s) located within a region of the protein spanning amino acids 70 to 92, thereby enhancing its DNA-binding activity in vitro. We report here that serine 83 appears to be the residue phosphorylated by CKII but that three other serines in this region can also be involved in phosphorylation and the enhancement of DNA-binding activity. A mutant that contained glutamate residues in place of these serines had only low-level binding activity; however, when the serines were replaced with glutamates and further mutations were made that increased the negative charge of the region, the resulting mutant showed a constitutively high level of binding equal to that achieved by phosphorylation of wild-type SRF. We have investigated the mechanism by which phosphorylation of SRF increases its DNA-binding activity. We have ruled out the possibilities that phosphorylation affects SRF dimerization or relieves inhibition due to masking of the DNA-binding domain by an amino-terminal region of the protein. Rather, using partial proteolysis to probe SRF's structure, we find that the conformation of SRF's DNA-binding domain is altered by phosphorylation. |
| Databáze: | OpenAIRE |
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