Suppression of long noncoding RNA LINC00324 restricts cell proliferation and invasion of papillary thyroid carcinoma through downregulation of TRIM29 via upregulating microRNA-195-5p
| Autor: | Qinghua Su, Jun Zhao, Jinkai Xu, Jiancang Ma, Zongyu Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Aging
endocrine system diseases Down-Regulation Biology medicine.disease_cause Thyroid carcinoma Downregulation and upregulation Cell Line Tumor microRNA medicine Gene silencing Humans Neoplasm Invasiveness Thyroid Neoplasms Cell Proliferation miR-195-5p Gene knockdown Cell growth TRIM29 Cell Biology Long non-coding RNA Up-Regulation DNA-Binding Proteins MicroRNAs Thyroid Cancer Papillary LINC00324 Cancer research papillary thyroid carcinoma RNA Long Noncoding Carcinogenesis Transcription Factors Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Long noncoding RNAs (lncRNAs) are identified as novel regulators of carcinogenesis. To date, the precise functions of lncRNAs in papillary thyroid carcinoma (PTC) remains poorly understood. The purposes of this work were to explore the potential relevance of lncRNA 00324 (LINC00324) in PTC. Levels of LINC00324 were markedly up-regulated in PTC. Silencing of LINC00324 significantly repressed the proliferation and invasion of PTC cells. LINC00324 was documented as a sponge of microRNA-195-5p (miR-195-5p). Decreased levels of miR-195-5p were detected in PTC. The up-regulation of miR-195-5p suppressed PTC cellular proliferation and invasion. Suppression of miR-195-5p partially reversed the LINC00324-knockdown-mediated effects in PTC cells. We identified tripartite motif-containing 29 (TRIM29) as a target gene of miR-195-5p. TRIM29 overexpression partially reversed the LINC00324-knockdown- or miR-195-5p-overexpression-mediated effects in PTC cells. In short, this work demonstrates that LINC00324 knockdown inhibits the proliferation and invasion of PTC cells by decreasing TRIM29 expression via up-regulating miR-195-5p expression. |
| Databáze: | OpenAIRE |
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