Ectopic expression of Arabidopsis L-type lectin receptor kinase genes LecRK-I.9 and LecRK-IX.1 in Nicotiana benthamiana confers Phytophthora resistance

Autor: Klaas Bouwmeester, David L. Nsibo, Yan Wang, Hagos M. Juhar, Francine Govers
Přispěvatelé: Sub Plant-Microbe Interactions, Plant Microbe Interactions
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Nicotiana benthamiana
Plant Science
01 natural sciences
Arabidopsis
Gene expression
Transgenes
Non-U.S. Gov't
Disease resistance
biology
Kinase
Research Support
Non-U.S. Gov't
Interfamily gene transfer
food and beverages
General Medicine
Plants
Plants
Genetically Modified
Biosystematiek
Original Article
Phytophthora
Phytophthora infestans
Transgene
Genetically Modified
Plant disease resistance
Research Support
Genes
Plant
03 medical and health sciences
Tobacco
Gene
Plant Diseases
Arabidopsis Proteins
fungi
Plant
biology.organism_classification
Molecular biology
Laboratorium voor Phytopathologie
030104 developmental biology
Genes
Laboratory of Phytopathology
LecRK
L-type lectin receptor kinases
Biosystematics
Ectopic expression
EPS
Agronomy and Crop Science
Protein Kinases
010606 plant biology & botany
Zdroj: Plant Cell Reports
Plant Cell, 35(4), 845. American Society of Plant Biologists
Plant Cell Reports (2016)
ISSN: 1432-203X
0721-7714
1040-4651
Popis: Key message Transgenic Nicotiana benthamiana lines with constitutive expression of an Arabidopsis lectin receptor kinase gene ( LecRK - I.9 or LecRK - IX.1 ) show enhanced resistance to Phytophthora pathogens, demonstrating conserved gene functionality after interfamily transfer. Abstract In plants, cell surface receptors mediate the first layer of innate immunity against pathogenic microbes. In Arabidopsis several L-type lectin receptor kinases (LecRKs) were previously found to function as Phytophthora resistance components. In this study, we determined the functionality of Arabidopsis LecRK-I.9 or LecRK-IX.1 in Phytophthora resistance when transferred into the Solanaceous plant Nicotiana benthamiana. Multiple transgenic lines were generated for each LecRK gene and molecular analyses revealed variation in transgene copy number, transgene expression levels and LecRK protein accumulation. Infection assays showed that transgenic N. benthamiana plants expressing either Arabidopsis LecRK-I.9 or LecRK-IX.1 are more resistant to Phytophthora capsici and to Phytophthora infestans. These results demonstrate that Arabidopsis LecRK-I.9 and LecRK-IX.1 retained their Phytophthora resistance function when transferred into N. benthamiana. Therefore, these LecRKs have the potential to function as a complementary Phytophthora resistance resource in distantly related plant species next to the canonical Phytophthora resistance genes encoding nucleotide-binding leucine-rich repeat proteins.
Databáze: OpenAIRE
Externí odkaz: