Rapid axonal sprouting and pruning accompany functional reorganization in primary visual cortex
| Autor: | Charles D. Gilbert, Justin N. J. McManus, Sally A. Marik, Winfried Denk, Homare Yamahachi |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Neuroscience(all) Green Fluorescent Proteins Presynaptic Terminals Visual system Biology Article Retina Statistics Nonparametric Lesion 03 medical and health sciences sysneuro 0302 clinical medicine Neuroplasticity medicine Animals Visual Pathways 030304 developmental biology Visual Cortex Neurons 0303 health sciences Brain Mapping Neuronal Plasticity General Neuroscience Anatomy Recovery of Function Axons Macaca fascicularis medicine.anatomical_structure Visual cortex nervous system Microscopy Fluorescence Receptive field Cerebral cortex medicine.symptom Visual Fields Neuroscience 030217 neurology & neurosurgery Sprouting |
| Zdroj: | Neuron |
| Popis: | The functional architecture of adult cerebral cortex retains a capacity for experience-dependent change. This is seen following focal binocular lesions, which induce rapid changes in receptive field size and position. To follow the dynamics of the circuitry underlying these changes, we imaged the intrinsic long-range horizontal connections within the lesion projection zone (LPZ) in adult macaque primary visual cortex. To image the same axons over time, we combined viral vector-mediated EGFP transfer and two-photon microscopy. The lesion triggered, within the first week, an approximately 2-fold outgrowth of axons toward the center of the LPZ. Over the subsequent month, axonal density declined due to a parallel process of pruning and sprouting but maintained a net increase relative to prelesion levels. The rate of turnover of axonal boutons also increased. The axonal restructuring recapitulates the pattern of exuberance and pruning seen in early development and correlates well with the functional changes following retinal lesions. |
| Databáze: | OpenAIRE |
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