ROR1 is highly expressed in circulating tumor cells and promotes invasion of pancreatic cancer
| Autor: | Wan‑Sheng Wang, Yun‑Hua Xu, Cai‑Fang Ni, Gui‑Li Xu, Jian Shen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Cancer Research Epithelial-Mesenchymal Transition Receptor Tyrosine Kinase-like Orphan Receptors Biochemistry Metastasis 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Cell Line Tumor Pancreatic cancer Biomarkers Tumor Genetics Humans Medicine Neoplasm Invasiveness Molecular Biology Gene knockdown Oncogene business.industry Cancer Middle Aged Cell cycle Neoplastic Cells Circulating medicine.disease Gene Expression Regulation Neoplastic Pancreatic Neoplasms 030104 developmental biology Oncology Gene Knockdown Techniques 030220 oncology & carcinogenesis ROR1 Cancer research Molecular Medicine Female business |
| Zdroj: | Molecular Medicine Reports. |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Pancreatic cancer (PaC) is an aggressive malignancy, which is associated with high levels of metastasis. Circulating tumor cells (CTCs), which may be considered a functional biomarker and promising treatment strategy for metastasis, are associated with the prognosis and progression of various metastatic cancers, including PaC. Receptor tyrosine kinase‑like orphan receptor 1 (ROR1) expression contributes to cell metastasis and poor clinical outcomes in malignant tumors. The present study aimed to explore the function of ROR1 in PaC CTCs. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to examine the expression of ROR1, E‑cadherin and N‑cadherin. Cell proliferative and invasive ability was assessed by MTT and Transwell assays, respectively. The results revealed that the mRNA and protein expression levels of ROR1 were augmented in PaC tissues. Furthermore, the mRNA expression levels of ROR1 were higher in CTCs compared with in peripheral blood cells, and ROR1 was more highly expressed in CTCs than in cells. Notably, CTCs exhibited a markedly greater proliferative and invasive capacity than PANC‑1 and SW‑1990 cells, whereas knockdown of endogenous ROR1 by small interfering RNA led to suppression of the invasion of CTCs. In addition, it was revealed that the mechanism underlying the effects of ROR1 on PaC CTC metastasis may involve the epithelial‑mesenchymal transition process. In conclusion, ROR1 may be considered a potential biomarker and therapeutic target for the treatment of PaC. |
| Databáze: | OpenAIRE |
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