Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection
| Autor: | Clícia Grativol Gaspar, Patricia Machado Bueno Fernandes, Paulo Cavalcanti Gomes Ferreira, David S. Buss, Paolla M. V. Abreu, José A. Ventura |
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| Rok vydání: | 2014 |
| Předmět: |
lcsh:Medicine
Plant Science Pathogenesis Pathology and Laboratory Medicine Bioinformatics Plant Viruses RNA interference Gene Expression Regulation Plant Molecular Cell Biology Databases Genetic Medicine and Health Sciences Plant defense against herbivory Arabidopsis thaliana lcsh:Science Phylogeny Expressed Sequence Tags Genetics Regulation of gene expression Multidisciplinary Carica Agriculture Viral Load Plants Host-Pathogen Interactions Epigenetics Genome Plant Research Article Proteasome Endopeptidase Complex DNA transcription Plant Pathogens Crops Biology Microbiology Fruits Molecular Genetics Virology Plant virus microRNA Gene Plant Diseases Biology and life sciences Base Sequence lcsh:R Organisms Cell Biology Plant Pathology Biotic stress biology.organism_classification Gene regulation Plant Leaves MicroRNAs lcsh:Q Gene expression Reactive Oxygen Species Sequence Alignment Viral Transmission and Infection |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 7, p e103401 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0103401 |
| Popis: | MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. |
| Databáze: | OpenAIRE |
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