Karyotype variability in Trypanosoma rangeli
| Autor: | Maria Rydåker, O E Sousa, Ulf Pettersson, Jan Henriksson, Aldo Solari |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Trypanosoma
Trypanosoma rangeli Trypanosoma brucei Species Specificity parasitic diseases Animals Humans Trypanosomatina Trypanosoma cruzi Genetics Polymorphism Genetic biology Genetic Variation Kinetoplastida Karyotype DNA Protozoan biology.organism_classification Molecular biology Electrophoresis Gel Pulsed-Field Infectious Diseases Karyotyping Animal Science and Zoology Parasitology DNA Probes Molecular probe |
| Zdroj: | Parasitology. 112:385-391 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYThe molecular karyotypes of several different protozoan parasites show high intra-species variation, including different kinetoplastids such as Trypanosoma brucei, Trypanosoma cruzi and Leishmania ssp. In this study, the molecular karyotype of Trypanosoma rangeli was examined. To evaluate potential intra-species molecular karyotype variations, 16 different samples were studied by pulsed field gel electrophoresis (PFGE) followed by ethidium bromide staining and hybridizations with 6 different probes. The result showed that different T. rangeli populations are highly polymorphic regarding the molecular karyotype, and thus suggests that PFGE analysis can be used for classification of different T. rangeli isolates. In addition, the molecular karyotype of T. rangeli was compared to molecular karyotypes of other kinetoplastids, and was shown to be distinctly different from that of T. cruzi, but shows some similarities with the karyotype described for T. brucei. Among the probes used one was identified as highly polymorphic, and thus informative for studies of different T. rangeli populations, and another was useful for differentiation between T. rangeli and T. cruzi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|