Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
| Autor: | Wendy Bernhard, Ayman El-Sayed, C. Ronald Geyer, Landon Pastushok, Humphrey Fonge, Carolina Gonzalez, Wayne Hill, Kris Barreto |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biodistribution Near-Infrared Fluorescence Imaging Indoles Phage display Receptor ErbB-3 Mice Nude Medicine (miscellaneous) Breast Neoplasms Immunoglobulin G law.invention near-infrared fluorescence imaging 03 medical and health sciences ErbB3 0302 clinical medicine HER3 law Cell Line Tumor Animals Humans Single-chain variable fragment Immunoglobulin Fragments Pharmacology Toxicology and Pharmaceutics (miscellaneous) Fluorescent Dyes antibody fragments biology Chemistry Benzenesulfonates Optical Imaging Molecular biology IRDye800CW Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Recombinant DNA biology.protein Heterografts Antibody Preclinical imaging Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | In vivo imaging is influenced by the half-life, tissue penetration, biodistribution, and affinity of the imaging probe. Immunoglobulin G (IgG) is composed of discrete domains with known functions, providing a template for engineering antibody fragments with desired imaging properties. Here, we engineered antibody-based imaging probes, consisting of different combinations of antibody domains, labeled them with the near-infrared fluorescent dye IRDye800CW, and evaluated their in vivo imaging properties. Antibody-based imaging probes were based on an anti-HER3 antigen binding fragment (Fab) isolated using phage display. Methods: We constructed six anti-HER3 antibody-based imaging probes: a single chain variable fragment (scFv), Fab, diabody, scFv-CH3, scFv-Fc, and IgG. IRDye800CW-labeled, antibody-based probes were injected into nude mice bearing FaDu xenografts and their distribution to the xenograft, liver, and kidneys was evaluated. Results: These imaging probes bound to recombinant HER3 and to the HER3-positive cell line, FaDu. Small antibody fragments with molecular weight |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|