Cell culture factors influencing in vitro expression of mouse mammary tumor virus
| Autor: | Lawrence J. T. Young, D. L. Fine, L. O. Arthur |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
viruses
Cell Plant Science Biology Virus Replication Dexamethasone Virus Cell Line Cell membrane Mice Viral Proteins Tissue culture medicine Animals Insulin Antigens Viral Mice Inbred C3H Cell growth Cell Membrane Mouse mammary tumor virus Temperature Mammary Neoplasms Experimental Drug Synergism RNA-Directed DNA Polymerase biology.organism_classification Molecular biology In vitro Culture Media Glucose medicine.anatomical_structure Mammary Tumor Virus Mouse Cell culture Biotechnology |
| Zdroj: | In Vitro. 12:693-701 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02797473 |
| Popis: | Several cell culture factors were found to influence in vitro expression of mouse mammary tumor virus (MMTV) in the mouse adenocarcinoma cell line Mm5mt/c1. Cells were propagated in a variety of commercially available cell culture media to which dexamethasone (DXM) was added as a stimulator of MMTV production. Culture seeding density, culture medium type, and glucose concentration each influenced MMTV production when expressed on a per cell basis. Maximum cell growth occurred in cultures grown in RPMI-1640 medium containing insulin. Those media which provided good cell growth were not necessarily optimal for virus expression. Addition of insulin did not stimulate MMTV synthesis although dexamethasone alone was stimulatory in all media used; however, maximum MMTV expression was obtained when dexamethasone and insulin were used in concert. Equivalent levels of MMTV-specific cell membrane antigen, MMTV-specific protein, and virus particles were produced at incubation temperatures of 32 degrees, 34 degrees or 37 degrees C; however, higher levels of virus-related RNA-dependent DNA polymerase (RDDP) activity were recovered from cultures incubated at 32 degrees and 34 degrees C than at 37 degrees C. Decreased levels of RDDP were attributed to enzyme thermolability at 37 degrees C incubation. |
| Databáze: | OpenAIRE |
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