Expansion of Bone Marrow Mesenchymal Stromal Cells in Perfused 3D Ceramic Scaffolds Enhances In Vivo Bone Formation
| Autor: | Allison I. Hoch, Nunzia Di Maggio, David Wendt, Ralph Duhr, Arne Mehrkens, Marcel Jakob |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Scaffold Ceramics Adolescent 0206 medical engineering Cell Culture Techniques Bone Marrow Cells 02 engineering and technology Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Young Adult Bioreactors In vivo Osteogenesis Bioreactor medicine Humans Ceramic Cell Proliferation Tissue Scaffolds Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Anatomy Middle Aged 020601 biomedical engineering In vitro Perfusion 030104 developmental biology medicine.anatomical_structure visual_art visual_art.visual_art_medium Molecular Medicine Polystyrene Bone marrow Biomedical engineering |
| Zdroj: | Biotechnology journal. 12(12) |
| ISSN: | 1860-7314 |
| Popis: | Bone marrow-derived mesenchymal stromal cells (BMSC), when expanded directly within 3D ceramic scaffolds in perfusion bioreactors, more reproducibly form bone when implanted in vivo as compared to conventional expansion on 2D polystyrene dishes/flasks. Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, we aimed to decouple the effects of specific parameters among these two model systems. We assessed the effects of the: i) 3D scaffold vs 2D surface, ii) ceramic vs polystyrene materials, and iii) BMSC niche established within the ceramic pores during in vitro culture, on subsequent in vivo bone formation. While BMSC expanded on 3D polystyrene scaffolds in the bioreactor could maintain their in vivo osteogenic potential, results were similar as BMSC expanded in monolayer on 2D polystyrene, suggesting little influence of the scaffold 3D environment. Bone formation was most reproducible when BMSC were expanded on 3D ceramic, highlighting the influence of the ceramic substrate. The presence of a pre-formed niche within the scaffold pores had negligible effects on the in vivo bone formation. The results of this study allow a greater understanding of the parameters required for perfusion bioreactor-based manufacturing of osteogenic grafts for clinical applications. |
| Databáze: | OpenAIRE |
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