Integrating silicon/zinc dual elements with PLGA microspheres in calcium phosphate cement scaffolds synergistically enhances bone regeneration
| Autor: | Xiaowen Sun, Xuehui Zhang, Yunyang Bai, Li Sun, Jing Zhang, Boon Chin Heng, Baowei Li, Min Gao, Xuliang Deng, Teliang Lu, Weiwei Liang, Jingsheng Lou, Jiandong Ye |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Calcium Phosphates
Silicon Bone Regeneration Surface Properties 0206 medical engineering Biomedical Engineering Plga microspheres chemistry.chemical_element macromolecular substances 02 engineering and technology Zinc Bone healing Mice chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Osteogenesis Materials Testing Animals General Materials Science Particle Size Bone regeneration Calcium phosphate cement Cells Cultured Tissue Scaffolds Bone Cements technology industry and agriculture Cell Differentiation General Chemistry General Medicine 021001 nanoscience & nanotechnology 020601 biomedical engineering Microspheres In vitro Rats PLGA RAW 264.7 Cells chemistry 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Materials Chemistry B. 8:3038-3049 |
| ISSN: | 2050-7518 2050-750X |
| Popis: | Integrating multiple pro-osteogenic factors into bone graft substitutes is a practical and effective approach to improve bone repair efficacy. Here, Si-Zn dual elements and PLGA microspheres were incorporated into calcium phosphate cement (CPC) scaffolds (PLGA/CPC-Si/Zn) as a novel strategy to synergistically enhance bone regeneration. The incorporation of PLGA microspheres and Si/Zn dual elements within CPC scaffolds improved the setting time, injectability and compressive strength. The PLGA/CPC-Si/Zn scaffolds displayed controlled sequential release of Si and Zn ions. In vitro, RAW 264.7 cells displayed the M2 phenotype with a high level of anti-inflammatory cytokines in response to PLGA/CPC-Si/Zn. The conditioned medium of RAW 264.7 cells cultured on the PLGA/CPC-Si/Zn scaffolds significantly enhanced the osteogenic differentiation of rat BMSCs. In a rat femur defect model, the implanted PLGA/CPC-Si/Zn scaffolds led to obvious new bone formation after 4 weeks, apparent bone ingrowth into the PLGA microspheres after 12 weeks, and was almost completely filled with mature new bone upon degradation of the PLGA microspheres at 24 weeks. These findings demonstrate that the PLGA/CPC-Si/Zn scaffolds promote osteogenesis by synergistically improving the immune microenvironment and biodegradability. Hence, integrating multiple trace elements together with degradable components within bone graft biomaterials can be an effective strategy for promoting bone regeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|