Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study
| Autor: | Rizal Rizal, Annisa Amalia, Meilinah Hidayat, Sijani Prahastuti, Wahyu Widowati, Rismawati Laila Qodariah, Hanna Sari Widya Kusuma, Satrio Wibowo, Stella Tinia Hasiana |
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| Přispěvatelé: | Penelitian Terapan Unggulan Perguruan Tinggi 2018 from Directorate General of Higher Education, Ministry of Research, Technology and Higher Education of the Republic of Indonesia. |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
cytotoxic fibronectin Reactive oxygen species Kidney medicine.diagnostic_test biology Mesangial cell Chemistry tgf-β1 lcsh:R chronic kidney disease ROS-DCFDA soybean TGF-β1 lcsh:Medicine medicine.disease Molecular biology Flow cytometry Fibronectin ros-dcfda medicine.anatomical_structure Glycine medicine biology.protein Kidney disease Transforming growth factor |
| Zdroj: | Majalah Obat Tradisional, Vol 24, Iss 3, Pp 160-168 (2019) Majalah Obat Tradisional; Vol 24, No 3 (2019); 160-168 |
| ISSN: | 2406-9086 1410-5918 |
| DOI: | 10.22146/mot.45053 |
| Popis: | Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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