14-3-3 Proteins and Other Candidates form Protein-Protein Interactions with the Cytosolic C-terminal End of SOS1 Affecting Its Transport Activity
| Autor: | Martin Jung, Cristina Martins Rodrigues, Kerstin Duscha, Joachim W. Deitmer, Richard Zimmermann, Maria Müller, H. Ekkehard Neuhaus, Ruth Wartenberg, Larry Fliegel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis 01 natural sciences lcsh:Chemistry Cytosol Gene Expression Regulation Plant Arabidopsis thaliana Protein Isoforms Biomass lcsh:QH301-705.5 Spectroscopy biology Chemistry Starch General Medicine Recombinant Proteins Computer Science Applications Cell biology Up-Regulation Protein Binding Sodium-Hydrogen Exchangers Proline Flowers DNA-binding protein Catalysis Article Protein–protein interaction Inorganic Chemistry 03 medical and health sciences Protein Domains ddc:570 Physical and Theoretical Chemistry Protein kinase A Molecular Biology membrane transporter salt tolerance Arabidopsis Proteins Organic Chemistry Cell Membrane Sodium salt-overly sensitive (SOS1) 14-3-3 proteins biology.organism_classification Plant Leaves 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 SOS1 010606 plant biology & botany Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 3334, p 3334 (2020) International Journal of Molecular Sciences Volume 21 Issue 9 |
| DOI: | 10.22028/d291-30941 |
| Popis: | The plasma membrane transporter SOS1 (SALT-OVERLY SENSITIVE1) is vital for plant survival under salt stress. SOS1 activity is tightly regulated, but little is known about the underlying mechanism. SOS1 contains a cytosolic, autoinhibitory C-terminal tail (abbreviated as SOS1 C-term), which is targeted by the protein kinase SOS2 to trigger its transport activity. Here, to identify additional binding proteins that regulate SOS1 activity, we synthesized the SOS1 C-term domain and used it as bait to probe Arabidopsis thaliana cell extracts. Several 14-3-3 proteins, which function in plant salt tolerance, specifically bound to and interacted with the SOS1 C-term. Compared to wild-type plants, when exposed to salt stress, Arabidopsis plants overexpressing SOS1 C-term showed improved salt tolerance, significantly reduced Na+ accumulation in leaves, reduced induction of the salt-responsive gene WRKY25, decreased soluble sugar, starch, and proline levels, less impaired inflorescence formation and increased biomass. It appears that overexpressing SOS1 C-term leads to the sequestration of inhibitory 14-3-3 proteins, allowing SOS1 to be more readily activated and leading to increased salt tolerance. We propose that the SOS1 C-term binds to previously unknown proteins such as 14-3-3 isoforms, thereby regulating salt tolerance. This finding uncovers another regulatory layer of the plant salt tolerance program. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|