SARS-CoV-2 Spatiotemporal Genomic and Molecular Analysis of the First Wave of the COVID-19 Pandemic in Macaé, the Brazilian Capital of Oil
| Autor: | Bruno da-Costa-Rodrigues, Caio Cheohen, Felipe Sciammarella, Allan Pierre-Bonetti-Pozzobon, Lupis Ribeiro, José Luciano Nepomuceno-Silva, Marcio Medeiros, Flávia Mury, Cintia Monteiro-de-Barros, Cristiano Lazoski, Manuela Leal-da-Silva, Amilcar Tanuri, Rodrigo Nunes-da-Fonseca |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
SARS-CoV-2
Organic Chemistry COVID-19 Genomics General Medicine epidemiology Macaé Brazil Catalysis Computer Science Applications Inorganic Chemistry Mutation Spike Glycoprotein Coronavirus Humans Angiotensin-Converting Enzyme 2 Physical and Theoretical Chemistry Pandemics Molecular Biology Phylogeny Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11497 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms231911497 |
| Popis: | The SARS-CoV-2 virus infection led to millions of deaths during the COVID-19 pandemic. Hundreds of workers from several other Brazilian cities, as well as from other countries, arrive daily in Macaé to work in the oil supply chain, making this city a putative hotspot for the introduction of new viral lineages. In this study, we performed a genomic survey of SARS-CoV-2 samples from Macaé during the first outbreak of COVID-19, combined with clinical data and a molecular integrative analysis. First, phylogenomic analyses showed a high occurrence of viral introduction events and the establishment of local transmissions in Macaé, including the ingression and spread of the B.1.1.28 lineage in the municipality from June to August 2020. Second, SARS-CoV-2 mutations were identified in patients with distinct levels of COVID-19 severity. Third, molecular interactions of the mutated spike protein from three B.1.1.33 local samples and human ACE2 showed higher interactions than that of the wild-type spike protein from the ancestral virus. Altogether, these results elucidate the SARS-CoV-2 genomic profile in a strategic Brazilian city and further explore the functional aspects of SARS-CoV-2 with a characterization of emerging viral mutations associated with clinical data and the potential targets for drug development against SARS-CoV-2. |
| Databáze: | OpenAIRE |
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