Modification of plasma membrane of differentiating preosseous chondrocytes: evidence for a degradative process in the mechanism of matrix vesicle formation
| Autor: | Piero Pollesello, Paola D'Andrea, Marco Martina, Benedetto de Bernard, Franco Vittur |
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| Přispěvatelé: | Pollesello, P, D'Andrea, Paola, Martina, M, DE BERNARD, B, Vittur, F. |
| Rok vydání: | 1990 |
| Předmět: |
Membrane Fluidity
Swine Biology Chondrocyte Extracellular matrix medicine Membrane fluidity Cyclic AMP Animals Growth Plate Cells Cultured chemistry.chemical_classification Membrane Glycoproteins Vesicle Cartilage Cell Membrane Membrane Proteins Cell Differentiation Cell Biology Alkaline Phosphatase Cell biology medicine.anatomical_structure Membrane Biochemistry chemistry Parathyroid Hormone Alkaline phosphatase Electrophoresis Polyacrylamide Gel Glycoprotein |
| Zdroj: | Experimental cell research. 188(2) |
| ISSN: | 0014-4827 |
| Popis: | Chondrocytes of the growth plate are differentiating cells. Their evolution leads to matrix vesicle formation and to cartilage mineralization. This is an in vitro study of the plasma membrane of Chondrocytes at two differentiation stages. Differences in protein and glycoprotein components, increased membrane fluidity, and responsiveness to PTH indicate that hypertrophic (“ossifying”) Chondrocytes possess a plasma membrane widely different from that of resting Chondrocytes. Their plasma membrane is particularly enriched in alkaline phosphatase (Mr 70K). Purified matrix vesicles contain the 70K form of alkaline phosphatase, but a 50K species is also detectable, a signal of degradative process. In fact, proteins and glycoproteins of matrix vesicles are less numerous than those of cell plasma membranes. It is suggested that, in vivo, matrix vesicle formation may be mediated by Ca2+-activated neutral proteases. |
| Databáze: | OpenAIRE |
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