Technical note: A histochemical approach in diagnosing hanging mechanical asphyxia on cadavers undergoing advanced putrefactive phenomena
| Autor: | Michele Boracchi, Francesca Maghin, Enrico Muccino, Francesca Maciocco, Guendalina Gentile, Salvatore Andreola, Riccardo Zoja |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
01 natural sciences Neck Injuries Asphyxia 03 medical and health sciences 0302 clinical medicine Cadaver Suicide Completed medicine Humans 030216 legal & forensic medicine Putrefaction Forensic Pathology Staining and Labeling business.industry 010401 analytical chemistry Technical note General Medicine Anatomy Middle Aged Elastic Tissue 0104 chemical sciences Elastic fibres Case-Control Studies Postmortem Changes Rehydration Solutions medicine.symptom business |
| Zdroj: | Medico-Legal Journal. 86:208-213 |
| ISSN: | 2042-1834 0025-8172 |
| Popis: | The authors used a particular protocol on putrefied corpses to highlight the cutaneous furrow. Two groups of cadavers were selected: in the first group (suicide by hanging mechanical asphyxia), the authors sampled cutaneous lozenges on the furrow still macroscopically recognisable, while in the second group (corpses deceased by different means), we sampled cutaneous lozenges in the hypostatic leaning. All specimens were divided in two: one underwent standard fixation while the other, previously rehydrated in Sandison’s solution, was then fixed in formalin. All specimens were stained with hematoxylin and eosin and Resorcin-Fuchsin. Samples processed with formalin and the H&E staining underlined various artifacts; vice versa, the Sandison solution associated with Resorcin-Fuchsin staining demonstrated the compression of the elastic fibres, with focal positivity in hanged corpses, and diffuse positivity in the entire hypostatic leaning. Sandison’s rehydrating solution and Resorcin-Fuchsin staining exalt the supposed application of an asphyctic mean even on putrefied corpses in those cases burdened by a diagnostic doubt. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|