Detection of PCV2e strains in Southeast China
| Autor: | Chunhua Wei, Jiayue Liu, Ke-Wei Fan, Man-Lin Luo, Ailing Dai, Xiaoyan Yang, Zhifeng Lin, Jianlin Fan, Jiankui Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Veterinary Medicine
0301 basic medicine Veterinary medicine Genotype Epidemiology animal diseases lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Genetic variation Amino acid alignment China Phylogenetic analysis General Neuroscience Strain (biology) lcsh:R virus diseases General Medicine New variant biology.organism_classification Recombination PCV2 Porcine circovirus Infectious Diseases 030104 developmental biology Herd General Agricultural and Biological Sciences |
| Zdroj: | PeerJ, Vol 6, p e4476 (2018) PeerJ |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.4476 |
| Popis: | Porcine circovirus 2 (PCV2) has been prevalent in swine herds in China since 2002, causing severe economic loss to the pig industry. The number of live pigs in southeast China is > 20 million. Since information on the genetic variation of PCV2 in the Fujian province is limited, the objective of the present work was to investigate the epidemiological and evolutionary characteristics of PCV2 in southeast China from 2013 to 2017. Of the 685 samples collected from 90 different swine herds from 2013 to 2017, 356 samples from 84 different swine herds were positive for PCV2. PCV2a, PCV2b, PCV2d, and PCV2e co-existed in the Fujian province, with PCV2d being the predominant circulating strain in swineherds and PCV2e being reported for the first time in China. Strikingly, PCV2-FJ-water DNA comes from contaminated river water and not infected animals. Sequence comparison among all isolates indicated that 95 isolates shared approximately 78.7%–100% nucleotide identity and 74.5%–100% amino acid identity for open reading frame 2 (ORF2). Amino acid alignment showed that the Cap protein of PCV2e differed markedly from those of PCV2a, PCV2b, PCV2c, and PCV2d. These results indicated that various PCV2 genotypes exist in China, and that PCV2 is continuously evolving, leading to rapid emergence of new variant stains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|