Comparison of corticosteroid- and L3T4+ antibody-immunosuppressed mouse models of Pneumocystis carinii pneumonia for evaluation of drugs and leukocytes
| Autor: | Richard S. Neiman, N. L. Bauer, W. L. Current, Marilyn S. Bartlett, Sherry F. Queener, Attilio Orazi, J. W. Smith |
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| Rok vydání: | 1994 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Microbiology (medical) Neutrophils medicine.medical_treatment Clinical Biochemistry Immunology chemical and pharmacologic phenomena Biology Dexamethasone Leukocyte Count Mice Immune system Antigen parasitic diseases medicine Animals Immunology and Allergy Immunosuppression Therapy Mice Inbred BALB C Pneumocystis Macrophages Pneumonia Pneumocystis Antibodies Monoclonal Immunosuppression medicine.disease Trimethoprim Disease Models Animal Pneumocystis carinii Evaluation Studies as Topic Monoclonal biology.protein Female Antibody Pneumonia (non-human) Research Article medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.1.5.511-516.1994 |
| Popis: | An immunologically immunosuppressed mouse model of Pneumocystis carinii pneumonia using antibody developed by Dialynas et al. (Immunol. Rev. 74:29-55, 1983) directed to L3T4+ T cells (referred to as L3T4+ antibody) was compared with a corticosteroid-immunosuppressed mouse model. Corticosteroid- or L3T4+ antibody-immunosuppressed BALB/c mice transtracheally inoculated with P. carinii developed severe infections within 5 weeks after inoculation and responded to treatments with an echinocandin B analog, LY302146, or trimethoprim plus sulfamethoxazole so that they had decreased numbers of P. carinii cysts and trophozoites. LY302146 appeared to be more effective in L3T4+ antibody-immunosuppressed mice than in dexamethasone-immunosuppressed mice. Leukocyte populations in lungs of both mouse models during development of infection and during treatment were compared by using immune cell-specific staining. Lungs of L3T4+ antibody-immunosuppressed mice had many more cells detected with pan-B antibody and pan-T antibody than dexamethasone-immunosuppressed mice and the lungs of successfully treated mice had about the same numbers of macrophages as those of nonimmunosuppressed uninfected mice. The immunologically immunosuppressed model will allow study of cytokines and other immune modulators alone and in combination with drugs. |
| Databáze: | OpenAIRE |
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