A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation
| Autor: | Manuel Alonso, Carlos A. Stella, Hilda I. Burgos, James R. Mattoon, Eugenia H. Ramos, Vanesa G. Pannunzio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Article Subject
Health Toxicology and Mutagenesis lcsh:Biotechnology Saccharomyces cerevisiae Antifungal drug lcsh:Medicine Biology Bioinformatics chemistry.chemical_compound symbols.namesake lcsh:TP248.13-248.65 Genetics Molecular Biology Growth medium Permease Endoplasmic reticulum lcsh:R Wild type General Medicine Golgi apparatus Brefeldin A biology.organism_classification chemistry Biophysics symbols Molecular Medicine Research Article Biotechnology |
| Zdroj: | Journal of Biomedicine and Biotechnology, Vol 2004, Iss 3, Pp 150-155 (2004) Journal of Biomedicine and Biotechnology |
| ISSN: | 1110-7243 |
| DOI: | 10.1155/S1110724304308077 |
| Popis: | The present work aims to develop a growth medium to render a wild-type strain ofSaccharomyces cerevisiaepermeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with3.0×10−3% SDS is employed. When Brefeldin A is added to this medium, a wild-type strain shows increased growth sensitivity and a diminished transport of the amino acid L-leucine. Since Brefeldin A exerts its effect on the endoplasmic reticulum and the Golgi apparatus, the medium permits the study of the drug effect on the intracellular traffic of L-leucine permeases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|